Knowledge transfer has a positive role in promoting the accumulation and creation of enterprise knowledge, and it is also one of the key ways to shape the competitive advantage of the entrepreneurial ecosystem. According to ecological theory, the enterprises in the entrepreneurial ecosystem are divided into two types of heterogeneous enterprises, symbiosis and competition, and a knowledge transfer model between heterogeneous enterprises is constructed. The general expression of the number of knowledge regeneration is derived from the model, and the balance point of knowledge transfer is obtained. The existence and stability of the system have been verified. Combined with numerical simulation, it is found that the frequency of inter-firm contact, the time of knowledge integration, and the proportion of the two types of enterprises will all affect the number of knowledge regeneration, which in turn affects the balance of knowledge transfer, and when the number of knowledge regeneration is greater than 1, the proportion of symbiotic enterprises will increase. As the number of knowledge reproduction increases, the activity of knowledge transfer within the system will also be enhanced; the increase in the degree of openness of the system can significantly promote knowledge transfer.
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