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Complex Systems and Complexity Science >

2018 , Vol. 15 >Issue 1: 11 - 17

DOI: https://doi.org/10.13306/j.1672-3813.2018.01.002

The Investigation of Molecular Mechanism of Guhong Injection Against Cerebral Ischemia-Reperfusion Injury in a Network Pharmacology Approach

  • FAN Fangfang ,
  • CHEN Shuman ,
  • YANG Hongjun ,
  • ZHANG Yi ,
  • TANG Shihuan ,
  • LI Defeng ,
  • ZHANG Yi ,
  • ZHANG Jingjing ,
  • GAO Jinhuan ,
  • FAN Fangfang ,
  • CHEN Shuman ,
  • YANG Hongjun ,
  • ZHANG Yi ,
  • TANG Shihuan ,
  • LI Defeng ,
  • ZHANG Yi ,
  • ZHANG Jingjing ,
  • GAO Jinhuan
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  • 1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2.College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 111137, China

Received date: 2018-02-12

  Online published: 2019-01-10

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Abstract

To investigate molecular mechanism of Guhong injection against rat cerebral ischemia-reperfusion injury, Middle Cerebral Artery Occlusion (MCAO) was adopted to evaluate the protection of Guhong injection against cerebral ischemia-reperfusion injury, and a network pharmacology approach was applied to investigate molecular mechanism of Guhong injection against cerebral ischemia-reperfusion injury. Guhong injection demonstrated effective protectionagainst cerebral ischemia-reperfusion injury was demonstrated. Based on the network pharmacology analysis, 131 compounds such as canthaxanthin A, γ-aminobutyric acid, regulated 12 functional pathways like amino acid metabolism, calcium signaling pathway, and had an effect on 64 key targets such as AGT, IL8 which was related with the Guhong injection-mediated protection. In this study, the network pharmacological analysis of Guhong-mediated protection provides potential clues for further research on the material basis and molecular mechanism.

Key words： Guhong injection; cerebral ischemia-reperfusion; network pharmacology; molecular mechanism

Cite this article

FAN Fangfang , CHEN Shuman , YANG Hongjun , ZHANG Yi , TANG Shihuan , LI Defeng , ZHANG Yi , ZHANG Jingjing , GAO Jinhuan , FAN Fangfang , CHEN Shuman , YANG Hongjun , ZHANG Yi , TANG Shihuan , LI Defeng , ZHANG Yi , ZHANG Jingjing , GAO Jinhuan . The Investigation of Molecular Mechanism of Guhong Injection Against Cerebral Ischemia-Reperfusion Injury in a Network Pharmacology Approach[J]. Complex Systems and Complexity Science, 2018 , 15(1) : 11 -17 . DOI: 10.13306/j.1672-3813.2018.01.002

References

[1]陈伟伟,高润霖,刘力生,等.《中国心血管病报告2016》概要[J].中国循环杂志,2017,32(6): 521530.
Chen Weiwei,Huang Zhiwei,Liu Lisheng,et al.Summary of China cardiovascular disease report 2016[J].Chinese Circulation Journal,2017,32(6): 521530.
[2]沈英生,黄智伟,黄金武.谷红注射液对脑缺血再灌注损伤的临床疗效[J].世界中医药,2016, 11(12):26372640.
Shen Yingsheng,Huang Zhiwei,Huang Jinwu.Clinical effect of guhong injection on cerebral ischemia reperfusion injury[J].World Chinese Medicine,2016, 11(12):26372640.
[3]张蕊,梁珍,杨楠,等.谷红注射液对脑缺血再灌注大鼠皮层血管内皮生长因子表达的影响[J].中国康复理论与实践,2015,21(7):770772.
Zhang Rui, Liang Zhen, Yang Nan.Effects of guhong injection on expression of vascular endothelial growth factor in cortex after cerebral isch-emia-reperfusion in rats[J].Chin J Rehabil Theory Pract, Jul,2015,21(7):770772.
[4]张蕊. 乙酰谷酰胺及谷红注射液抗脑缺血再灌注损伤的作用机制研究[D].北京:北京协和医学院,2015.
Zhang Rui.Studies on the neuroprotective effects and mechanism of aceglutamide and Guhong injection on cerebral ischemia-reperfusion injury[D].Beijing: Peking Union Medical College,2015.
[5]舒明春,万海同,周惠芬,等.谷红注射液抗脑缺血性再灌注损伤的作用及其机制[J].中国中药杂志,2014,39(24):48294833.
Shu Mingchun,Wan Haitong, Zhou Huifen,et al.Effect and mechanism of Guhong injection against cerebral ischemia reperfusion injury[J]. China Journal of CHinese Materia Media,2014,39(24):48294833.
[6]何晓玮,范晓萍,钟涛,等.谷红注射液治疗急性脑梗死总有效率和安全性的meta分析[J].中华中医药学刊,2014,32(11):26022605.
He Xiaowei,Fan Xiaoping,Zhong Tao,et al. Meta-analysis of guhong injection on acute cerebral infarction[J].Chinese Archives Traditional Chinese Medicine, 2014,32(11):26022605.
[7]张茁.谷红注射液治疗急性脑梗死有效性和安全性的多中心、随机、开放性临床观察[J].现代预防医学,2010,37(22):43824383,4385.
Zhang Zhuo.Multi-center,randomized and open clinical study on the efficacy and safety of Guhong injection in patients with acute cerebral infarction[J].Modern Preventive Medicine,2010,37(22):43824383,4385.
[8]李宵,吴茵,支旭然,等.UPLC-MS/MS同时测定谷红注射液中7种有效成分[J].中国实验方剂学杂志,2017,23(21):6469.
Li Xiao,Wu Yin,Zhi Xuran,et al.Simultaneous determination of seven active components in guhong injection[J].Chinese Journal of Experimental Traditional Medical Formulae, 2017,23(21):6469.
[9]刘世军,唐志书,崔春利,等.中药红花化学成分的研究进展[J].河南中医,2017,37(1):168171.
Liu Shijun,Tang Zhishu,Cui Chunli,et al.Research progress of the chemical components of chinese herb honghua (flos carthami)[J].Henan Traditional Chinese Medicine,2017,37(1):168171.
[10] 瞿城,乐世俊,林航,等.红花化学成分研究[J].中草药,2015,46(13):18721877.
Qu Cheng,Yue Shijun,Lin Hang,et al.Chemical constituents of Carthamus tinctorius[J].CHinese Traditional and Herbal Drugs,2015,46(13):18721877.
[11] 何昱,周惠芬,黄丽娜,等.HPLC-DAD法同时测定谷红注射液中7个组分的含量[J].药物分析杂志,2015,35(6):954959.
He Yu,Zhou Huifen,Huang Lina,et al.Stimultaneous quantificatiion of seven components in Guhong injection by HPLC-DAD[J].Chin J Pharm Anal,2015,35(6):954959.
[12] 乐世俊. 红花化学成分研究与活性评价[D].南京: 南京中医药大学,2015.
Le Shijun.The study on chemical constituents and pharmacological activities of Carthamus tinctorius[D].Nanjing: Nanjing University of Chinese Medicine,2015.
[13] Famakin B M.The immune response to acute focal cferebral ischemia and associated post-stroke immunodepression: a focused review[J].Aging Dis, 2014,5(5): 307326.
[14] Kawabori M, Yenari M A. Inflammatory responses in brain ischemia[J].Curr Med Chem, 2015, 22(10): 12581277.
[15] Li H L, Xie Y C, Zhang N N,et al.Disruption of IP3R2-mediated Ca2+ signaling pathway in astrocytes ameliorates neuronal death and brain damage while reducing behavioral deficits after focal ischemic stroke[J].Cell Calcium, 2015,58(6): 565576.
[16] Yan C L, Zhang J, Wang S,et al.Neuroprotective effects of rutaecarpine on cerebral ischemia reperfusion injury[J].Neural Regen Res, 2013, 8(22): 20302038.
[17] Rink C, Khanna S. Significance of brain tissue oxygenation and the arachidonic acid cascade in stroke[J].Antioxid Redox Signal, 2011,14(10): 18891903.
[18] Demerlé pallardy C, Gillard roubert V, Marin J G,et al.In vitro antioxidant neuroprotective activity of BN 80933, a dual inhibitor of neuronal nitric oxide synthase and lipid peroxidation[J]. Neurochem, 2000,74(5):207986.
[19] 冯璐,王明洋,刘俊雅,等.谷红注射液对脑缺血大鼠大脑皮质线粒体功能的改善作用[J].医学研究杂志,2017,46(1):2730.
Feng Lu,Wang Mingyang,Liu Junya,et al.Effect of gu hong injection on the function of cerebral cortex mitochondria in rats with cerebral ischemia[J].J Med Res,2017,46(1):2730.
[20] 胡学强.注射用乙酰谷酰胺的研究进展[J].中国处方药,2017,15(1):1719.
Hu Xueqiang.Advances in aceglutamide for injection research[J]. Journal of China Prescription Drug ,2017,15(1):1719.
[21] 孙达,常静,林莉莉.红花黄色素对实验性脑缺血的保护作用[J].时珍国医国药,2017,28(11):26362638.
Sun Da,Chang Jing,Lin Lili,et al.The protective effect of safflower yellow pigment on experimental cerebral ischemia[J].Lishizhen Medicine and Mate Riamedica Research,2017,28(11):26362638.
[22] 姜华.羟基红花黄色素A对大鼠脑缺血再灌注损伤的保护作用及机制[J].中药材,2013,36(3):462464.
Jang Hua.Effect and mechanism of hydroxysafflower yellow A on cerebral ischemia-reperfusion injury in rats[J].Journal of Chinese Medicinal Materials,2013, 36(3):462464.
[23] 姚炜,库宝善,张疆寅,等.L盐酸赖氨酸对局部脑缺血大鼠的治疗作用[J].药学学报,1999,(6):25.
Yao Wei,Ku Baoshan,Zhang Jiangyu,et al.The therapeutic effect of L-Lysine Hydrochloride against cerebral ischemia rats[J].Acta Pharmaceutica Sinica,1999,(6):25.
[24] 高丽. 血管紧张素(17)在高血压及脑缺血损伤中的作用及机制研究[D].南京: 南京医科大学,2013.
Gao Li.The neuroprotective role of Ang-(1-7) in hypertension and ischemic injury and its related mechanisms[D].Nanjing:Nanjing Medical University,2013.
[25] 吴锦萍,张春兵.中药有效成分保护脑缺血再灌注损伤的研究进展[J].中国老年学杂志,2015,14(35):40684050.
Wu Jinping,Zhang Chunbing.The research progress of effective components of traditional Chinese medicine against cerebral ischemia reperfusion injury[J].Chinese journal of gerontology,2015,14(35):40684050.
[26] Garcia-Bonilla L,Benakis C,Moore J,et al. Immune mechanisms in cer-ebral ischemic tolerance[J]. Front Neurosci,2014,8( 44) : 3389.
[27] 方正旭. ET1eNOS基因多态性与江_省略_压及硝苯地平降压疗效的相关性研究[D].南昌: 南昌大学第二附属医院,2016.
Fang Zhengxu.Association of ET-1and eNOS polymoephisms with essential hypertention and antihypertensive effect of Nifedipine in Chinese population of Jiangxi province[D].Nanchang: Second Affiliated Hospital of Nanchang University,2016.
[28] 李敏.黄芪甲苷对大鼠脑缺血再灌注损伤后血脑屏障及脑的保护作用机制研究[D].西安:第四军医大学,2012.
Li MIN.Study on the protective mechanism of astragaloside in the blood brain barrier and brain after cerebral ischemia reperfusion injury[D]. Xi′an: Fourth Military Medical University,2012.
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