The Investigation of Molecular Mechanism of Guhong Injection Against Cerebral Ischemia-Reperfusion Injury in a Network Pharmacology Approach
FAN Fangfang1,2, CHEN Shuman1, YANG Hongjun1, ZHANG Yi2, TANG Shihuan1, LI Defeng1, ZHANG Yi1, ZHANG Jingjing1, GAO Jinhuan1
1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; 2.College of Ethnic Medicine, Chengdu University of Traditional Chinese Medicine, Chengdu 111137, China
Abstract：To investigate molecular mechanism of Guhong injection against rat cerebral ischemia-reperfusion injury, Middle Cerebral Artery Occlusion (MCAO) was adopted to evaluate the protection of Guhong injection against cerebral ischemia-reperfusion injury, and a network pharmacology approach was applied to investigate molecular mechanism of Guhong injection against cerebral ischemia-reperfusion injury. Guhong injection demonstrated effective protectionagainst cerebral ischemia-reperfusion injury was demonstrated. Based on the network pharmacology analysis, 131 compounds such as canthaxanthin A, γ-aminobutyric acid, regulated 12 functional pathways like amino acid metabolism, calcium signaling pathway, and had an effect on 64 key targets such as AGT, IL8 which was related with the Guhong injection-mediated protection. In this study, the network pharmacological analysis of Guhong-mediated protection provides potential clues for further research on the material basis and molecular mechanism.
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